Rapid europium-sensitized fluorescent determination of ulifloxacin, the active metabolite of prulifloxacin, in human serum and urine
نویسندگان
چکیده
A new fluorescent method was developed based on the ulifloxacin-europium (III)-sodium dodecylbenzene sulfonate system for the determination of ulifloxacin, the active metabolite of prulifloxacin. Sodium dodecylbenzene sulfonate formed a ternary complex with ulifloxacin-europium (III) and significantly enhaneed the characteristic fluorescence of europium (III). The enhanced fluorescence intensity showed a good linear relationship with the concentration of ulifloxacin in the range of 5.0 × 10-8 - 2.0 × 10-6M with a detection limit of 2.0 × 10-10 M (3σ). This method is rapid and sensitive, and has been successfully applied to the determination of ulifloxacin in human urine and serum samples.
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